DNA polymerases with improved reverse transcriptase activity
نویسندگان
چکیده
منابع مشابه
Fluorometric Assay for DNA Polymerases and Reverse Transcriptase
We report a quick, easy and inexpensive fluorometric assay that measures the activity of replication enzymes using PicoGreen. The systems tested include replication of the natural template M13 Gori by E. coli DNA polymerase III holoenzyme and the replication of a synthetic homopolymer by human immunodeficiency virus reverse transcriptase. A direct comparison of the fluorometric assay with the ...
متن کاملFluorometric assay for DNA polymerases and reverse transcriptase.
We report a quick, easy and inexpensive fluorometric assay that measures the activity of replication enzymes using Pico-GreenTM. The systems tested include replication of the natural template M13 Gori by E. coli DNA polymerase III holoenzyme and the replication of a synthetic homopolymer by human immunodeficiency virus reverse transcriptase. A direct comparison of the fluorometric assay with th...
متن کاملKinetic analysis of reverse transcriptase activity of bacterial family A DNA polymerases.
Some bacterial thermostable, wild-type or genetically engineered family A DNA polymerases have reverse transcriptase activity. However, difference in reverse transcriptase activities of family A DNA polymerases and retroviral reverse transcriptases (RTs) is unclear. In this study, comparative kinetic analysis was performed for the reverse transcriptase activities of the wild-type enzyme of fami...
متن کاملNon-radioactive screening of DNA polymerases by using oligo r(A-U) as primer/template for reverse transcriptase activity.
polymerase (lane e), and Thermococcus litoralis-derived Vent@ DNA polymerase (lane f) synthesized poly d(A-T), while Pyrococcus sp・-derived PyrobestTM DNA polymerase (lane g) failed to synthesize poly d(A-T). ThoughBcaBESTTM DNA polymerase was known to have RT activity (including Takara's RT-PCR kit), the RT activities of KOD and Vent@ DNA polymerases have not been yet reported. It should be me...
متن کاملEvolving thermostable reverse transcriptase activity in a DNA polymerase scaffold.
DNA polymerases are involved in all DNA synthesis that occurs in nature. Furthermore, DNA polymerases are the workhorses in numerous important molecular biological core technologies like the widely applied polymerase chain reaction (PCR), cDNA cloning, genome sequencing, and diagnostics based on nucleic acids. In each of these processes the DNA polymerase requires a DNA strand as a template for...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2009
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.23.1_supplement.482.4